DMRT1 Mouse Monoclonal Antibody [Clone ID: OTI2H1]

CAT#: CF807746

Carrier-free (BSA/glycerol-free) DMRT1 mouse monoclonal antibody, clone OTI2H1 (formerly 2H1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DMRT1 (RC208108, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DMRT1 (1:2000). Positive lysates LY411862 (100ug) and LC411862 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-DMRT1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DMRT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807746) (1:150)

Specifications

Product Data

• Clone Name: OTI2H1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-210 of human DMRT1(NP_068770) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.3 kDa
• Gene Name: Homo sapiens doublesex and mab-3 related transcription factor 1 (DMRT1), transcript variant 1, mRNA.
• Database Link:
  NP_068770
  Entrez Gene 1761 Human
  UniProt ID Q9Y5R6
• Background: This gene is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes. This gene exhibits a gonad-specific and sexually dimorphic expression pattern. Defective testicular development and XY feminization occur when this gene is hemizygous. [provided by RefSeq, Jul 2008]
• Synonyms: CT154; DMT1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors