SIRP alpha (SIRPA) Mouse Monoclonal Antibody [Clone ID: OTI7B3]

CAT#: CF807751

Carrier-free (BSA/glycerol-free) SIRPA mouse monoclonal antibody, clone OTI7B3 (formerly 7B3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRPA (RC222380, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRPA (1:2000). Positive lysates LY421878 (100ug) and LC421878 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807751) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807751) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807751) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807751) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SIRPA mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807751) (1:150)

• Flow cytometric analysis of living 293T cells transfected with SIRPA overexpression plasmid (RC222380), Red)/empty vector (PS100001, Blue) using anti-SIRPA antibody (TA807751). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

• Flow cytometric analysis of living ACHN cells, using anti-SIRPA antibody (TA807751, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

• Flow cytometric analysis of living A375 cells, using anti-SIRPA antibody (TA807751, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

• Flow cytometric analysis of living SK-MEL-28 cells, using anti-SIRPA antibody (TA807751, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

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Specifications

Product Data

• Clone Name: OTI7B3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SIRPA (NP_001035111) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens signal regulatory protein alpha (SIRPA), transcript variant 1, mRNA.
• Database Link:
  NP_001035111
  Entrez Gene 140885 Human
  UniProt ID P78324
• Background: The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: BIT; CD172A; MFR; MYD-1; P84; PTPNS1; SHPS1; SIRP

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Transmembrane