BAMBI Mouse Monoclonal Antibody [Clone ID: OTI5D5]

CAT#: CF807759

Carrier-free (BSA/glycerol-free) BAMBI mouse monoclonal antibody, clone OTI5D5 (formerly 5D5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAMBI (RC200195, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BAMBI (1:2000). Positive lysates LY415799 (100ug) and LC415799 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5D5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 21-152 of human BAMBI(NP_036474) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.6 kDa
• Gene Name: Homo sapiens BMP and activin membrane bound inhibitor (BAMBI), mRNA.
• Database Link:
  NP_036474
  Entrez Gene 68010 MouseEntrez Gene 83837 RatEntrez Gene 25805 Human
  UniProt ID Q13145
• Background: This gene encodes a transmembrane glycoprotein related to the type I receptors of the transforming growth factor-beta (TGF-beta) family, whose members play important roles in signal transduction in many developmental and pathological processes. The encoded protein however is a pseudoreceptor, lacking an intracellular serine/threonine kinase domain required for signaling. Similar proteins in frog, mouse and zebrafish function as negative regulators of TGF-beta, which has led to the suggestion that the encoded protein may function to limit the signaling range of the TGF-beta family during early embryogenesis. [provided by RefSeq, Jul 2008]
• Synonyms: NMA

Reference Data

• Protein Families: Transmembrane