CGK2 (PRKG2) Mouse Monoclonal Antibody [Clone ID: OTI15E6]

CAT#: CF807769

Carrier-free (BSA/glycerol-free) PRKG2 mouse monoclonal antibody,clone OTI15E6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKG2 (RC215879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKG2 (1:2000). Positive lysates LY401884 (100ug) and LC401884 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI15E6
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 40-320 of human PRKG2 (NP_006250) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 87.3 kDa
• Gene Name: Homo sapiens protein kinase cGMP-dependent 2 (PRKG2), transcript variant 1, mRNA.
• Database Link:
  NP_006250
  Entrez Gene 19092 MouseEntrez Gene 25523 RatEntrez Gene 5593 Human
  UniProt ID Q13237
• Background: This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein plays a role in the regulation of fluid balance in the intestine. A similar protein in mouse is thought to regulate differentiation and proliferation of cells in the colon. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
• Synonyms: cGK2; cGKII; PRKGR2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, Long-term depression, Olfactory transduction