BTG1 Mouse Monoclonal Antibody [Clone ID: OTI7H6]

CAT#: CF807834

Carrier-free (BSA/glycerol-free) BTG1 mouse monoclonal antibody, clone OTI7H6 (formerly 7H6)

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BTG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807834)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BTG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807834)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BTG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807834)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BTG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807834)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BTG1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807834)

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Specifications

Product Data

• Clone Name: OTI7H6
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BTG1 (NP_001722) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19 kDa
• Gene Name: Homo sapiens BTG anti-proliferation factor 1 (BTG1), mRNA.
• Database Link:
  NP_001722
  Entrez Gene 12226 MouseEntrez Gene 29618 RatEntrez Gene 694 Human
  UniProt ID P62324
• Background: This gene is a member of an anti-proliferative gene family that regulates cell growth and differentiation. Expression of this gene is highest in the G0/G1 phases of the cell cycle and downregulated when cells progressed through G1. The encoded protein interacts with several nuclear receptors, and functions as a coactivator of cell differentiation. This locus has been shown to be involved in a t(8;12)(q24;q22) chromosomal translocation in a case of B-cell chronic lymphocytic leukemia. [provided by RefSeq, Oct 2008]
• Synonyms: APRO2

Reference Data

• Protein Families: Transcription Factors