IGF1 Receptor (IGF1R) Mouse Monoclonal Antibody [Clone ID: OTI4C4]

CAT#: CF807835

Carrier-free (BSA/glycerol-free) IGF1R mouse monoclonal antibody, clone OTI4C4 (formerly 4C4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF1R (RC214928, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF1R (1:2000). Positive lysates LY400311 (100ug) and LC400311 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 cell lines lysates by using anti-IGF1R monoclonal antibody (1:500).

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  Living HEK293T cells transfected with either RC214928 overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-IGF1R antibody (TA807835), and then analyzed by flow cytometry (1:100).

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  Flow cytometric analysis of living HepG2 cells, using anti-IGF1R antibody (TA807835), (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:25).

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  Flow cytometric analysis of living A549 cells, using anti-IGF1R antibody (TA807835), (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:25).

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Specifications

Product Data

• Clone Name: OTI4C4
• Applications: FC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 741-935 of human IGF1R(NP_000866) produced in Insect.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81 kDa
• Gene Name: Homo sapiens insulin like growth factor 1 receptor (IGF1R), transcript variant 1, mRNA.
• Database Link:
  NP_000866
  Entrez Gene 16001 MouseEntrez Gene 25718 RatEntrez Gene 3480 Human
  UniProt ID P08069
• Background: This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May
• Synonyms: CD221; IGFIR; IGFR; JTK13

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Adherens junction, Colorectal cancer, Endocytosis, Focal adhesion, Glioma, Long-term depression, Melanoma, Oocyte meiosis, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer