BOB1 (POU2AF1) Mouse Monoclonal Antibody [Clone ID: OTI2D3]

CAT#: CF807930

Carrier-free (BSA/glycerol-free) POU2AF1 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1 (1:2000). Positive lysates LY416779 (100ug) and LC416779 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POU2AF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807930) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human tonsil using anti-POU2AF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, TA807930) (1:200)

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  Flow cytometric Analysis of penetrated Ramos cells, using anti-POU2AF1 antibody (TA807930), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue). The right is Jurkat cells as negtive control (1:100).

Specifications

Product Data

• Clone Name: OTI2D3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human POU2AF1(NP_006226) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.3 kDa
• Gene Name: Homo sapiens POU class 2 homeobox associating factor 1 (POU2AF1), mRNA.
• Database Link:
  NP_006226
  Entrez Gene 18985 MouseEntrez Gene 690528 RatEntrez Gene 5450 Human
  UniProt ID Q16633
• Background: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. [UniProtKB/Swiss-Prot Function]
• Synonyms: BOB1; OBF-1; OBF1; OCAB