SP110 Mouse Monoclonal Antibody [Clone ID: OTI2A1]

CAT#: CF807938

Carrier-free (BSA/glycerol-free) SP110 mouse monoclonal antibody, clone OTI2A1 (formerly 2A1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SP110 (RC205648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SP110. Positive lysates LY417943 (100ug) and LC417943 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807938)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807938)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807938)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807938)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807938)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807938)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SP110 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807938)

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Specifications

Product Data

• Clone Name: OTI2A1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 232-521 of human SP110(NP_004501) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61.7 kDa
• Gene Name: Homo sapiens SP110 nuclear body protein (SP110), transcript variant b, mRNA.
• Database Link:
  NP_004501
  Entrez Gene 3431 Human
  UniProt ID Q9HB58
• Background: The nuclear body is a multiprotein complex that may have a role in the regulation of gene transcription. This gene is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. In addition, it has been suggested that the protein may play a role in ribosome biogenesis and in the induction of myeloid cell differentiation. Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified. [provided by RefSeq, Jul
• Synonyms: IFI41; IFI75; IPR1; VODI

Reference Data

• Protein Families: Druggable Genome, Transcription Factors