GM CSF (CSF2) Mouse Monoclonal Antibody [Clone ID: OTI6A3]

CAT#: CF807985

Carrier-free (BSA/glycerol-free) CSF2 mouse monoclonal antibody,clone OTI6A3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF2 (RC211109, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF2 (1:500). Positive lysates LY400257 (100ug) and LC400257 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807985) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807985) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807985) (1:150)

Specifications

Product Data

• Clone Name: OTI6A3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 18-144 of human CSF2(NP_000749) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 14.4 kDa
• Gene Name: Homo sapiens colony stimulating factor 2 (CSF2), mRNA.
• Database Link:
  NP_000749
  Entrez Gene 1437 Human
  UniProt ID P04141
• Background: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
• Synonyms: GMCSF

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway