FOLR2 Mouse Monoclonal Antibody [Clone ID: OTI4G6]

CAT#: CF808026

Carrier-free (BSA/glycerol-free) FOLR2 mouse monoclonal antibody, clone OTI4G6 (formerly 4G6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOLR2 (RC212993, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOLR2 (1:500). Positive lysates LY400280 (100ug) and LC400280 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FOLR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808026) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FOLR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808026) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FOLR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808026) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FOLR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808026) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-FOLR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808026) (1:150)

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Specifications

Product Data

• Clone Name: OTI4G6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 20-221 of human FOLR2(NP_000794) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.1 kDa
• Gene Name: Homo sapiens folate receptor beta (FOLR2), transcript variant 1, mRNA.
• Database Link:
  NP_000794
  Entrez Gene 2350 Human
  UniProt ID P14207
• Background: The protein encoded by this gene is a member of the folate receptor (FOLR) family, and these genes exist in a cluster on chromosome 11. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and they mediate delivery of 5-methyltetrahydrofolate to the interior of cells. This protein has a 68% and 79% sequence homology with the FOLR1 and FOLR3 proteins, respectively. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. Multiple transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: BETA-HFR; FBP; FR-BETA; FR-P3; PL-1

Reference Data

• Protein Families: Druggable Genome, Secreted Protein