G protein alpha 16 (GNA15) Mouse Monoclonal Antibody [Clone ID: OTI6B3]

CAT#: CF808136

Carrier-free (BSA/glycerol-free) GNA15 mouse monoclonal antibody, clone OTI6B3 (formerly 6B3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GNA15 (Cat# RC202243, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNA15 (Cat# TA808136)(1:2000). Positive lysates LY419560 (100ug) and LC419560 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6B3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-256 of human GNA15(NP_002059) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.4 kDa
• Gene Name: G protein subunit alpha 15
• Database Link:
  NP_002059
  Entrez Gene 89788 RatEntrez Gene 2769 Human
  UniProt ID P30679
• Synonyms: GNA16

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway