ATP dependent metalloprotease YME1L1 (YME1L1) Mouse Monoclonal Antibody [Clone ID: OTI4E3]

CAT#: CF808146

Carrier-free (BSA/glycerol-free) YME1L1 mouse monoclonal antibody, clone OTI4E3 (formerly 4E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY YME1L1 (RC203167, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-YME1L1 (1:2000). Positive lysates LY408345 (100ug) and LC408345 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4E3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 26-345 of human YME1L1(NP_647474) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83.1 kDa
• Gene Name: Homo sapiens YME1-like 1 (S. cerevisiae) (YME1L1), nuclear gene encoding mitochondrial protein, transcript variant 2
• Database Link:
  NP_647474
  Entrez Gene 10730 Human
  UniProt ID Q96TA2
• Background: The protein encoded by this gene is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
• Synonyms: FTSH; MEG4; PAMP; YME1L

Reference Data

• Protein Families: Druggable Genome, Protease