Cyclin Y (CCNY) Mouse Monoclonal Antibody [Clone ID: OTI12F10]

CAT#: CF808174

Carrier-free (BSA/glycerol-free) CCNY mouse monoclonal antibody, clone OTI12F10 (formerly 12F10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCNY (RC220991, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCNY (1:2000).

Specifications

Product Data

• Clone Name: OTI12F10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CCNY (NP_859049) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens cyclin Y (CCNY), transcript variant 2, mRNA.
• Database Link:
  NP_859049
  Entrez Gene 67974 MouseEntrez Gene 361261 RatEntrez Gene 219771 Human
  UniProt ID Q8ND76
• Background: Cyclins, such as CCNY, control cell division cycles and regulate cyclin-dependent kinases (e.g., CDC2; MIM 116940) (Li et al., 2009 [PubMed 18060517]). [supplied by OMIM, May 2009]
• Synonyms: C10orf9; CBCP1; CCNX; CFP1