CDC42 binding protein kinase alpha (CDC42BPA) Mouse Monoclonal Antibody [Clone ID: OTI12C12]

CAT#: CF808221

Carrier-free (BSA/glycerol-free) CDC42BPA mouse monoclonal antibody, clone OTI12C12 (formerly 12C12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC42BPA (RC222587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDC42BPA (1:500). Positive lysates LY415030 (100ug) and LC415030 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI12C12
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1419-1638 of human CDC42BPA(NP_055641) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 185.9 kDa
• Gene Name: Homo sapiens CDC42 binding protein kinase alpha (CDC42BPA), transcript variant A, mRNA.
• Database Link:
  NP_055641
  Entrez Gene 114116 RatEntrez Gene 226751 MouseEntrez Gene 8476 Human
  UniProt ID Q5VT25
• Background: The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq, Jul 2008]
• Synonyms: MRCK; MRCKA; PK428

Reference Data

• Protein Families: Druggable Genome, Protein Kinase