TRBP (TARBP2) Mouse Monoclonal Antibody [Clone ID: OTI2C12]

CAT#: CF808239

Carrier-free (BSA/glycerol-free) TARBP2 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TARBP2 (RC201043, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TARBP2 (1:2000). Positive lysates LY418167 (100ug) and LC418167 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TARBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808239) (1:150)

Specifications

Product Data

• Clone Name: OTI2C12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TARBP2 (NP_004169) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.7 kDa
• Gene Name: Homo sapiens TARBP2 subunit of RISC loading complex (TARBP2), transcript variant 3, mRNA.
• Database Link:
  NP_004169
  Entrez Gene 21357 MouseEntrez Gene 363006 RatEntrez Gene 6895 Human
  UniProt ID Q15633
• Synonyms: LOQS; TRBP; TRBP1; TRBP2

Reference Data

• Protein Families: Stem cell - Pluripotency