Prostate Specific Antigen (KLK3) Mouse Monoclonal Antibody [Clone ID: OTI2F3]

CAT#: CF808335

Carrier-free (BSA/glycerol-free) KLK3 mouse monoclonal antibody, clone OTI2F3 (formerly 2F3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLK3 (RC202740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLK3 (1:2000). Positive lysates LY419823 (100ug) and LC419823 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KLK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808335) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-KLK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808335) (1:150)

Specifications

Product Data

• Clone Name: OTI2F3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 25-261 of human KLK3(NP_001639) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.8 kDa
• Gene Name: Homo sapiens kallikrein related peptidase 3 (KLK3), transcript variant 1, mRNA.
• Database Link:
  NP_001639
  Entrez Gene 354 Human
  UniProt ID P07288
• Background: Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
• Synonyms: APS; hK3; KLK2A1; PSA

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Pathways in cancer, Prostate cancer