MRP5 (ABCC5) Mouse Monoclonal Antibody [Clone ID: OTI6C6]

CAT#: CF808370

Carrier-free (BSA/glycerol-free) ABCC5 mouse monoclonal antibody, clone OTI6C6 (formerly 6C6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCC5 (RC217669, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCC5 (1:2000). Positive lysates LY422609 (100ug) and LC422609 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6C6
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ABCC5(NP_001018881) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.5 kDa
• Gene Name: Homo sapiens ATP binding cassette subfamily C member 5 (ABCC5), transcript variant 2, mRNA.
• Database Link:
  NP_001018881
  Entrez Gene 27416 MouseEntrez Gene 116721 RatEntrez Gene 10057 Human
  UniProt ID O15440
• Background: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. This protein may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site is unclear. [provided by RefSeq, Jul 2008]
• Synonyms: ABC33; EST277145; MOAT-C; MOATC; MRP5; pABC11; SMRP

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters