RGL2 Mouse Monoclonal Antibody [Clone ID: OTI10F2]

CAT#: CF808391

Carrier-free (BSA/glycerol-free) RGL2 mouse monoclonal antibody, clone OTI10F2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RGL2 (RC207582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGL2 (1:2000). Positive lysates LY417774 (100ug) and LC417774 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

• Immunohistochemical staining of paraffin-embedded Human tonsil using anti-RGL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808391) (1:150)

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Specifications

Product Data

• Clone Name: OTI10F2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 547-777 of human RGL2(NP_004752) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83.4 kDa
• Gene Name: Homo sapiens ral guanine nucleotide dissociation stimulator like 2 (RGL2), transcript variant 1, mRNA.
• Database Link:
  NP_004752
  Entrez Gene 5863 Human
  UniProt ID O15211
• Background: Interacts with SAMD9. [UniProtKB/Swiss-Prot Function]
• Synonyms: HKE1.5; KE1.5; RAB2L

Reference Data

• Protein Families: Druggable Genome