p90 Autoantigen (KIAA1524) Mouse Monoclonal Antibody [Clone ID: OTI9E6]

CAT#: CF808455

Carrier-free (BSA/glycerol-free) KIAA1524 mouse monoclonal antibody, clone OTI9E6 (formerly 9E6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIAA1524 (RC219918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIAA1524 (1:2000). Positive lysates LY412250 (100ug) and LC412250 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KIAA1524 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808455) (1:150)

Specifications

Product Data

• Clone Name: OTI9E6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 635-905 of human KIAA1524(NP_065941) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 102 kDa
• Gene Name: Homo sapiens cell proliferation regulating inhibitor of protein phosphatase 2A (CIP2A), mRNA.
• Database Link:
  NP_065941
  Entrez Gene 57650 Human
  UniProt ID Q8TCG1
• Synonyms: CIP2A; p90

Reference Data

• Protein Families: Druggable Genome