MARK4 Mouse Monoclonal Antibody [Clone ID: OTI9B7]

CAT#: CF808507

Carrier-free (BSA/glycerol-free) MARK4 mouse monoclonal antibody, clone OTI9B7 (formerly 9B7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MARK4 (RC222580, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK4. Positive lysates LY403112 (100ug) and LC403112 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MARK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808507) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-MARK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808507) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MARK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808507) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MARK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808507) (1:150)

Show all 5 images

Specifications

Product Data

• Clone Name: OTI9B7
• Applications: IHC, WB
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 390-467 of human MARK4(NP_113605) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens microtubule affinity regulating kinase 4 (MARK4), transcript variant 2, mRNA.
• Database Link:
  NP_113605
  Entrez Gene 232944 MouseEntrez Gene 57787 Human
  UniProt ID Q96L34
• Background: This gene encodes a member of the microtubule affinity-regulating kinase family. These protein kinases phosphorylate microtubule-associated proteins and regulate the transition between stable and dynamic microtubules. The encoded protein is associated with the centrosome throughout mitosis and may be involved in cell cycle control. Expression of this gene is a potential marker for cancer, and the encoded protein may also play a role in Alzheimer's disease. Pseudogenes of this gene are located on both the short and long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
• Synonyms: MARK4L; MARK4S; MARKL1; MARKL1L; PAR-1D

Reference Data

• Protein Families: Druggable Genome, Protein Kinase