NAP1L3 Mouse Monoclonal Antibody [Clone ID: OTI3G3]

CAT#: CF808549

Carrier-free (BSA/glycerol-free) NAP1L3 mouse monoclonal antibody, clone OTI3G3 (formerly 3G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NAP1L3 (RC205297, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAP1L3 (1:2000). Positive lysates LY417930 (100ug) and LC417930 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NAP1L3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808549) (1:150)

Specifications

Product Data

• Clone Name: OTI3G3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-315 of human NAP1L3(NP_004529) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 57.4 kDa
• Gene Name: Homo sapiens nucleosome assembly protein 1 like 3 (NAP1L3), mRNA.
• Database Link:
  NP_004529
  Entrez Gene 4675 Human
  UniProt ID Q99457
• Background: This gene is intronless and encodes a member of the nucleosome assembly protein (NAP) family. This gene is linked closely to a region of genes responsible for several X-linked mental retardation syndromes. [provided by RefSeq, Dec 2010]
• Synonyms: MB20; NPL3