IRS1 Mouse Monoclonal Antibody [Clone ID: OTI3G10]

CAT#: CF808553

Carrier-free (BSA/glycerol-free) IRS1 mouse monoclonal antibody, clone OTI3G10 (formerly 3G10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRS1 (RC208765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRS1 (1:2000). Positive lysates LY417237 (100ug) and LC417237 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-IRS1 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IRS1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808553) (1:150)

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Specifications

Product Data

• Clone Name: OTI3G10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-280 of human IRS1(NP_005535) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 131.4 kDa
• Gene Name: Homo sapiens insulin receptor substrate 1 (IRS1), mRNA.
• Database Link:
  NP_005535
  Entrez Gene 16367 MouseEntrez Gene 25467 RatEntrez Gene 3667 Human
  UniProt ID P35568
• Background: This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]
• Synonyms: HIRS-1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Insulin signaling pathway, Neurotrophin signaling pathway, Type II diabetes mellitus