VAMP5 Mouse Monoclonal Antibody [Clone ID: OTI7F2]

CAT#: CF808605

Carrier-free (BSA/glycerol-free) VAMP5 mouse monoclonal antibody,clone OTI7F2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VAMP5 (RC204973, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VAMP5. Positive lysates LY416522 (100ug) and LC416522 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-VAMP5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808605) (1:500)

Specifications

Product Data

• Clone Name: OTI7F2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VAMP5 (NP_006625) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.6 kDa
• Gene Name: Homo sapiens vesicle associated membrane protein 5 (VAMP5), mRNA.
• Database Link:
  NP_006625
  Entrez Gene 10791 Human
  UniProt ID O95183
• Background: Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of vesicles and cell membranes. The VAMP5 gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. This VAMP family member may participate in vesicle trafficking events that are associated with myogenesis. [provided by RefSeq, Jul 2008]
• Synonyms: MYOBREVIN; myobrevin; vesicle-associated membrane protein 5; vesicle-associated membrane protein 5 (myobrevin)

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport