FOXO4 Mouse Monoclonal Antibody [Clone ID: OTI5A5]

CAT#: CF808678

Carrier-free (BSA/glycerol-free) FOXO4 mouse monoclonal antibody, clone OTI5A5 (formerly 5A5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXO4 (RC213185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXO4 (1:2000). Positive lysates LY401798 (100ug) and LC401798 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-FOXO4 monoclonal antibody (1:200).

Specifications

Product Data

• Clone Name: OTI5A5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 206-505 of human FOXO4 (NP_005929) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: Homo sapiens forkhead box O4 (FOXO4), transcript variant 1, mRNA.
• Database Link:
  NP_005929
  Entrez Gene 54601 MouseEntrez Gene 302415 RatEntrez Gene 4303 Human
  UniProt ID P98177
• Background: This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
• Synonyms: AFX; AFX1; MLLT7

Reference Data

• Protein Families: Transcription Factors