PARG Mouse Monoclonal Antibody [Clone ID: OTI9F6]

CAT#: CF808712

Carrier-free (BSA/glycerol-free) PARG mouse monoclonal antibody, clone OTI9F6 (formerly 9F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PARG (RC208530, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARG (1:2000). Positive lysates LY418533 (100ug) and LC418533 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI9F6
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-367 of human PARG(NP_003622) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 110.9 kDa
• Gene Name: Homo sapiens poly(ADP-ribose) glycohydrolase (PARG), transcript variant 1, mRNA.
• Database Link:
  NP_003622
  Entrez Gene 26430 MouseEntrez Gene 8505 Human
• Background: Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. [provided by RefSeq, Jul 2008]
• Synonyms: PARG99