VAPB Mouse Monoclonal Antibody [Clone ID: OTI4C8]

CAT#: CF808758

Carrier-free (BSA/glycerol-free) VAPB mouse monoclonal antibody, clone OTI4C8 (formerly 4C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VAPB (RC200517, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VAPB (1:2000). Positive lysates LY401498 (100ug) and LC401498 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4C8
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-223 of human VAPB(NP_004729) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27 kDa
• Gene Name: Homo sapiens VAMP associated protein B and C (VAPB), transcript variant 1, mRNA.
• Database Link:
  NP_004729
  Entrez Gene 56491 MouseEntrez Gene 60431 RatEntrez Gene 9217 Human
  UniProt ID O95292
• Background: The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking. [provided by RefSeq, Jul 2008]
• Synonyms: ALS8; VAMP-B; VAP-B

Reference Data

• Protein Families: Transmembrane