PLCXD1 Mouse Monoclonal Antibody [Clone ID: OTI2D7]

CAT#: CF808765

Carrier-free (BSA/glycerol-free) PLCXD1 mouse monoclonal antibody, clone OTI2D7 (formerly 2D7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLCXD1 (RC202835, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLCXD1. Positive lysates LY413089 (100ug) and LC413089 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PLCXD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808765) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PLCXD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808765) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PLCXD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808765) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PLCXD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808765) (1:150)

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PLCXD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808765) (1:150)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PLCXD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808765) (1:150)

Show all 7 images

Specifications

Product Data

• Clone Name: OTI2D7
• Applications: IHC, WB
• Recommended Dilution: IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PLCXD1(NP_060860) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.5 kDa
• Gene Name: Homo sapiens phosphatidylinositol specific phospholipase C X domain containing 1 (PLCXD1), transcript variant 1, mRNA.
• Database Link:
  NP_060860
  Entrez Gene 55344 Human
  UniProt ID Q9NUJ7
• Background: This gene is the most terminal protein-coding gene in the pseudoautosomal (PAR) region on chromosomes X and Y. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
• Synonyms: LL0XNC01-136G2.1