Gemin 2 (GEMIN2) Mouse Monoclonal Antibody [Clone ID: OTI6G4]

CAT#: CF808823

Carrier-free (BSA/glycerol-free) SIP1 mouse monoclonal antibody, clone OTI6G4 (formerly 6G4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIP1 (RC211219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIP1 (1:2000). Positive lysates LY418547 (100ug) and LC418547 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6G4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SIP1 (NP_003607) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31.4 kDa
• Gene Name: Homo sapiens gem nuclear organelle associated protein 2 (GEMIN2), transcript variant alpha, mRNA.
• Database Link:
  NP_003607
  Entrez Gene 66603 MouseEntrez Gene 84404 RatEntrez Gene 8487 Human
  UniProt ID O14893
• Background: This gene encodes one of the proteins found in the SMN complex, which consists of several gemin proteins and the protein known as the survival of motor neuron protein. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. [provided by RefSeq, Aug 2011]
• Synonyms: SIP1; SIP1-delta

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency