SOX6 Mouse Monoclonal Antibody [Clone ID: OTI5E4]

CAT#: CF808936

Carrier-free (BSA/glycerol-free) SOX6 mouse monoclonal antibody,clone OTI5E4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX6 (RC212046, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX6. (1:1000) (1:2000). Positive lysates LY403243 (100ug) and LC403243 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5E4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-244 of human SOX6(NP_201583) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 89.6 kDa
• Gene Name: Homo sapiens SRY-box transcription factor 6 (SOX6), transcript variant 2, mRNA.
• Database Link:
  NP_201583
  Entrez Gene 20679 MouseEntrez Gene 293165 RatEntrez Gene 55553 Human
  UniProt ID P35712
• Background: This gene encodes a member of the D subfamily of sex determining region y-related transcription factors that are characterized by a conserved DNA-binding domain termed the high mobility group box and by their ability to bind the minor groove of DNA. The encoded protein is a transcriptional activator that is required for normal development of the central nervous system, chondrogenesis and maintenance of cardiac and skeletal muscle cells. The encoded protein interacts with other family members to cooperatively activate gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009]
• Synonyms: HSSOX6; SOXD

Reference Data

• Protein Families: Transcription Factors