DECR1 Mouse Monoclonal Antibody [Clone ID: OTI5D6]

CAT#: CF808947

Carrier-free (BSA/glycerol-free) DECR1 mouse monoclonal antibody,clone OTI5D6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DECR1 (RC211257, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DECR1 (1:2000). Positive lysates LY419973 (100ug) and LC419973 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DECR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808947) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DECR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808947) (1:150)

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  Flow cytometric Analysis of permeabilized Hela cells, using anti-DECR1 antibody (TA808947), (Red), compared to negative control (PBS), (Blue) (1:100).

Specifications

Product Data

• Clone Name: OTI5D6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DECR1 (NP_001350) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.1 kDa
• Gene Name: Homo sapiens 2,4-dienoyl-CoA reductase 1 (DECR1), transcript variant 1, mRNA.
• Database Link:
  NP_001350
  Entrez Gene 1666 Human
  UniProt ID Q16698
• Background: This gene encodes an accessory enzyme which participates in the beta-oxidation and metabolism of unsaturated fatty enoyl-CoA esters. [provided by RefSeq, Jul 2008]
• Synonyms: DECR; NADPH; SDR18C1

Reference Data

• Protein Families: Druggable Genome