C7orf16 (PPP1R17) Mouse Monoclonal Antibody [Clone ID: OTI2A8]

CAT#: CF808959

Carrier-free (BSA/glycerol-free) C7ORF16 mouse monoclonal antibody,clone OTI2A8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C7ORF16 (RC207007, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C7ORF16 (1:2000). Positive lysates LY416501 (100ug) and LC416501 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2A8
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C7ORF16 (NP_006649) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens protein phosphatase 1 regulatory subunit 17 (PPP1R17), transcript variant 1, mRNA.
• Database Link:
  NP_006649
  Entrez Gene 10842 Human
  UniProt ID O96001
• Background: The protein encoded by this gene is found primarily in cerebellar Purkinje cells, where it functions as a protein phosphatase inhibitor. The encoded protein is a substrate for cGMP-dependent protein kinase. An allele of this gene was discovered that increases susceptibility to hypercholesterolemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
• Synonyms: C7orf16; GSBS

Reference Data

• Protein Pathways: Long-term depression