RAB3IP Mouse Monoclonal Antibody [Clone ID: OTI4A7]

CAT#: CF808962

Carrier-free (BSA/glycerol-free) RAB3IP mouse monoclonal antibody,clone OTI4A7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB3IP (RC218153, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAB3IP (1:2000). Positive lysates LY411671 (100ug) and LC411671 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-RAB3IP monoclonal antibody (HeLa: human; 293T: human;A431: human) (1:500).

Specifications

Product Data

• Clone Name: OTI4A7
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RAB3IP (NP_071901) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.1 kDa
• Gene Name: Homo sapiens RAB3A interacting protein (RAB3IP), transcript variant alpha 1, mRNA.
• Database Link:
  NP_071901
  Entrez Gene 29885 RatEntrez Gene 216363 MouseEntrez Gene 117177 Human
  UniProt ID Q96QF0
• Synonyms: RABIN3; RABIN8