PD L2 (PDCD1LG2) Mouse Monoclonal Antibody [Clone ID: OTI7B10]

CAT#: CF808990

Carrier-free (BSA/glycerol-free) PDCD1LG2 mouse monoclonal antibody, clone OTI7B10 (formerly 7B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1LG2 (RC224141, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1LG2 (1:2000). Positive lysates LY410802 (100ug) and LC410802 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PDCD1LG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808990) (1:150).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PDCD1LG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808990) (1:150).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PDCD1LG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808990) (1:150).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PDCD1LG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808990) (1:150) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PDCD1LG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808990) (1:150).

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PDCD1LG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808990) (1:150) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PDCD1LG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808990) (1:150).

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PDCD1LG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808990) (1:150).

• Flow cytometric analysis of living 293T cells transfected with PDL2 overexpression plasmid (RC224141, Red)/empty vector (PS100001, Blue) using anti-PDL2 antibody (TA808990). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

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Specifications

Product Data

• Clone Name: OTI7B10
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDCD1LG2 (NP_079515) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.8 kDa
• Gene Name: Homo sapiens programmed cell death 1 ligand 2 (PDCD1LG2), mRNA.
• Database Link:
  NP_079515
  Entrez Gene 80380 Human
  UniProt ID Q9BQ51
• Background: Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. [UniProtKB/Swiss-Prot Function]
• Synonyms: B7DC; bA574F11.2; Btdc; CD273; PD-L2; PDCD1L2; PDL2

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)