Cyclophilin F (PPIF) Mouse Monoclonal Antibody [Clone ID: OTI7D12]

CAT#: CF809030

Carrier-free (BSA/glycerol-free) PPIF mouse monoclonal antibody, clone OTI7D12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPIF (RC223397, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPIF (1:2000). Positive lysates LY417109 (100ug) and LC417109 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from mouse heart tissue lysate by using anti-PPIF monoclonal antibody (1:500).

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-PPIF monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PPIF-Knockout 293T cells (KO, Cat# LC811541) were separated by SDS-PAGE and immunoblotted with anti-PPIF monoclonal antibody TA809030, (1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809030) (1:1000)

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Specifications

Product Data

• Clone Name: OTI7D12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPIF(NP_005720) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.8 kDa
• Gene Name: Homo sapiens peptidylprolyl isomerase F (PPIF), mRNA.
• Database Link:
  NP_005720
  Entrez Gene 105675 MouseEntrez Gene 282819 RatEntrez Gene 10105 Human
  UniProt ID P30405
• Background: The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]
• Synonyms: Cyp-D; CyP-M; CYP3; CypD