BNP (NPPB) Mouse Monoclonal Antibody [Clone ID: OTI3H2]

CAT#: CF809065

Carrier-free (BSA/glycerol-free) NPPB mouse monoclonal antibody, clone OTI3H2 (formerly 3H2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPPB (RC206590, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPPB (1:2000). Positive lysates LY400900 (100ug) and LC400900 (20ug) can be purchased separately from OriGene.

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  NPPB (BNP/NT-ProBNP) Luminex ELISA with 3H2 Capture (TA809065) and 6F8 Detection (TA809024) Antibodies. Substrate used: Recombinant Human BNP/NT-ProBNP domain expressed in E.coli.

Specifications

Product Data

• Clone Name: OTI3H2
• Applications: LMNX, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 27-102 of human NPPB (NP_002512) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 11.9 kDa
• Gene Name: Homo sapiens natriuretic peptide B (NPPB), mRNA.
• Database Link:
  NP_002512
  Entrez Gene 4879 Human
  UniProt ID P16860
• Background: This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Jul 2008]
• Synonyms: BNP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Stem cell - Pluripotency