Estrogen Related Receptor alpha (ESRRA) Mouse Monoclonal Antibody [Clone ID: OTI3G10]

CAT#: CF809108

Carrier-free (BSA/glycerol-free) ESRRA mouse monoclonal antibody, clone OTI3G10 (formerly 3G10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ESRRA (RC209028, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ESRRA (1:2000). Positive lysates LY417978 (100ug) and LC417978 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3G10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 206-417 of human ESRRA (NP_004442) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46 kDa
• Gene Name: Homo sapiens estrogen related receptor alpha (ESRRA), transcript variant 1, mRNA.
• Database Link:
  NP_004442
  Entrez Gene 2101 Human
  UniProt ID P11474
• Background: The protein encoded by this gene is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site-specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium-chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. A processed pseudogene of ESRRA is located on chromosome 13q12.1. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]
• Synonyms: ERR1; ERRa; ERRalpha; ESRL1; NR3B1

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors