Alkaline Phosphatase (ALPI) Mouse Monoclonal Antibody [Clone ID: OTI3C1]

CAT#: CF809178

Carrier-free (BSA/glycerol-free) ALPI mouse monoclonal antibody,clone OTI3C1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALPI (RC224178, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALPI (1:2000). Positive lysates LY400612 (100ug) and LC400612 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ALPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809178) (1:500)

Specifications

Product Data

• Clone Name: OTI3C1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALPI (NP_001622) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.7 kDa
• Gene Name: Homo sapiens alkaline phosphatase, intestinal (ALPI), mRNA.
• Database Link:
  NP_001622
  Entrez Gene 248 Human
  UniProt ID P09923
• Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq, Jul 2008]
• Synonyms: IAP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways