GRB7 Mouse Monoclonal Antibody [Clone ID: OTI15E7]

CAT#: CF809284

Carrier-free (BSA/glycerol-free) GRB7 mouse monoclonal antibody,clone OTI15E7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRB7 (RC215362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRB7 (1:2000). Positive lysates LY401639 (100ug) and LC401639 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 5 different cell lines and mouse spleen tissue lysate by using anti-GRB7 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI15E7
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GRB7 (NP_005301) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.5 kDa
• Gene Name: Homo sapiens growth factor receptor bound protein 7 (GRB7), transcript variant 1, mRNA.
• Database Link:
  NP_005301
  Entrez Gene 14786 MouseEntrez Gene 84427 RatEntrez Gene 2886 Human
  UniProt ID Q14451
• Background: The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
• Synonyms: growth factor receptor-bound protein 7; OTTHUMP00000164352

Reference Data

• Protein Families: Druggable Genome, Embryonic stem cells, Stem cell - Pluripotency