TBX20 Mouse Monoclonal Antibody [Clone ID: OTI5D4]

CAT#: CF809303

Carrier-free (BSA/glycerol-free) TBX20 mouse monoclonal antibody,clone OTI5D4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBX20 (RC212412, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TBX20 (1:2000). Positive lysates LY421469 (100ug) and LC421469 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5D4
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-340 of human TBX20(NP_001071121) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.1 kDa
• Gene Name: Homo sapiens T-box transcription factor 20 (TBX20), transcript variant 1, mRNA.
• Database Link:
  NP_001071121
  Entrez Gene 57246 MouseEntrez Gene 57057 Human
  UniProt ID Q9UMR3
• Background: This gene encodes a T-box family member. The T-box family members share a common DNA binding domain, termed the T-box, and they are transcription factors involved in the regulation of developmental processes. This gene is essential for heart development. Mutations in this gene are associated with diverse cardiac pathologies, including defects in septation, valvulogenesis and cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
• Synonyms: ASD4

Reference Data

• Protein Families: Transcription Factors