RING1 Mouse Monoclonal Antibody [Clone ID: OTI3G7]

CAT#: CF809319

Carrier-free (BSA/glycerol-free) RING1 mouse monoclonal antibody,clone OTI3G7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RING1 (RC202650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RING1 (1:2000). Positive lysates LY401023 (100ug) and LC401023 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RING1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RING1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809319) (1:1000)

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Specifications

Product Data

• Clone Name: OTI3G7
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 88-362 of human RING1(NP_002922) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.2 kDa
• Gene Name: Homo sapiens ring finger protein 1 (RING1), mRNA.
• Database Link:
  NP_002922
  Entrez Gene 19763 MouseEntrez Gene 309626 RatEntrez Gene 6015 Human
  UniProt ID Q06587
• Background: This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq, Jul 2008]
• Synonyms: RING1A; RNF1

Reference Data

• Protein Families: Druggable Genome, Transcription Factors