TGF beta Receptor III (TGFBR3) Mouse Monoclonal Antibody [Clone ID: OTI4F11]

CAT#: CF809351

Carrier-free (BSA/glycerol-free) TGFBR3 mouse monoclonal antibody,clone OTI4F11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TGFBR3 (RC216595, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TGFBR3 (1:2000). Positive lysates LY401117 (100ug) and LC401117 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TGFBR3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809351) (1:500)

Specifications

Product Data

• Clone Name: OTI4F11
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 531-777 of human TGFBR3(NP_003234) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 93.3 kDa
• Gene Name: Homo sapiens transforming growth factor beta receptor 3 (TGFBR3), transcript variant 1, mRNA.
• Database Link:
  NP_003234
  Entrez Gene 7049 Human
  UniProt ID Q03167
• Background: This locus encodes the transforming growth factor (TGF)-beta type III receptor. The encoded receptor is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Sep 2010]
• Synonyms: betaglycan; BGCAN

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane