n-Myc (MYCN) Mouse Monoclonal Antibody [Clone ID: OTI1B5]

CAT#: CF809363

Carrier-free (BSA/glycerol-free) MYCN mouse monoclonal antibody,clone OTI1B5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYCN (RC201241, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYCN (1:2000). Positive lysates LY417334 (100ug) and LC417334 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1B5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 229-464 of human MYCN(NP_005369) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.4 kDa
• Gene Name: Homo sapiens MYCN proto-oncogene, bHLH transcription factor (MYCN), transcript variant 2, mRNA.
• Database Link:
  NP_005369
  Entrez Gene 4613 Human
  UniProt ID P04198
• Background: This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
• Synonyms: bHLHe37; MODED; N-myc; NMYC; ODED

Reference Data

• Protein Families: Druggable Genome, Transcription Factors