Eph receptor A6 (EPHA6) Mouse Monoclonal Antibody [Clone ID: OTI5B9]

CAT#: CF809373

Carrier-free (BSA/glycerol-free) EPHA6 mouse monoclonal antibody,clone OTI5B9

Product Images

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EPHA6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:500).

Specifications

Product Data

• Clone Name: OTI5B9
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 225-481 of human EPHA6 (NP_001073917) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 126.4 kDa
• Gene Name: Homo sapiens EPH receptor A6 (EPHA6), transcript variant 1, mRNA.
• Database Link:
  NP_001073917
  Entrez Gene 13840 MouseEntrez Gene 29202 RatEntrez Gene 285220 Human
  UniProt ID Q9UF33
• Background: Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. [UniProtKB/Swiss-Prot Function]
• Synonyms: EHK-2; EHK2; EK12; EPA6; HEK12; PRO57066

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance