RAB23 Mouse Monoclonal Antibody [Clone ID: OTI2A8]

CAT#: CF809380

Carrier-free (BSA/glycerol-free) RAB23 mouse monoclonal antibody,clone OTI2A8

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAB23 (RC221508, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAB23 (1:2000). Positive lysates LY405187 (100ug) and LC405187 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 7 different cell lines by using anti-RAB23 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RAB23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809380) (1:2000)

Specifications

Product Data

• Clone Name: OTI2A8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-234 of human RAB23 (NP_899050) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.5 kDa
• Gene Name: Homo sapiens RAB23, member RAS oncogene family (RAB23), transcript variant 2, mRNA.
• Database Link:
  NP_899050
  Entrez Gene 19335 MouseEntrez Gene 367242 RatEntrez Gene 51715 Human
  UniProt ID Q9ULC3
• Background: This gene encodes a small GTPase of the Ras superfamily. Rab proteins are involved in the regulation of diverse cellular functions associated with intracellular membrane trafficking, including autophagy and immune response to bacterial infection. The encoded protein may play a role in central nervous system development by antagonizing sonic hedgehog signaling. Disruption of this gene has been implicated in Carpenter syndrome as well as cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
• Synonyms: HSPC137

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Hedgehog signaling pathway