RhoGAP (ARHGAP5) Mouse Monoclonal Antibody [Clone ID: OTI9H2]

CAT#: CF809437

Carrier-free (BSA/glycerol-free) ARHGAP5 mouse monoclonal antibody,clone OTI9H2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP5 (RC217203, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP5 (1:2000).

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  Western blot analysis of extracts (35ug) from 8 different cell lines by using anti-ARHGAP5 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI9H2
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 948-1261 of human ARHGAP5 (NP_001164) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens Rho GTPase activating protein 5 (ARHGAP5), transcript variant 2, mRNA.
• Database Link:
  NP_001164
  Entrez Gene 11855 MouseEntrez Gene 299012 RatEntrez Gene 394 Human
  UniProt ID Q13017
• Background: Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: GFI2; p190-B; p190BRhoGAP; RhoGAP5

Reference Data

• Protein Pathways: Focal adhesion, Leukocyte transendothelial migration