RRAS Mouse Monoclonal Antibody [Clone ID: OTI2B7]

CAT#: CF809505

Carrier-free (BSA/glycerol-free) RRAS mouse monoclonal antibody,clone OTI2B7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RRAS (RC204820, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RRAS (1:2000). Positive lysates LY416758 (100ug) and LC416758 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and RRAS-Knockout HeLa cells (KO, Cat# LC831537) were separated by SDS-PAGE and immunoblotted with anti-RRAS monoclonal antibody TA809505 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

Specifications

Product Data

• Clone Name: OTI2B7
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human RRAS (NP_006261) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.3 kDa
• Gene Name: Homo sapiens RAS related (RRAS), mRNA.
• Database Link:
  NP_006261
  Entrez Gene 361568 RatEntrez Gene 6237 Human
  UniProt ID P10301
• Synonyms: Oncogene RRAS; related RAS viral (r-ras) oncogene homolog

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway, Regulation of actin cytoskeleton, Tight junction