ZCCHC17 Mouse Monoclonal Antibody [Clone ID: OTI1F11]

CAT#: CF809515

Carrier-free (BSA/glycerol-free) ZCCHC17 mouse monoclonal antibody,clone OTI1F11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZCCHC17 (RC208652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZCCHC17 (1:2000). Positive lysates LY413942 (100ug) and LC413942 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ZCCHC17 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI1F11
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ZCCHC17 (NP_057589) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.4 kDa
• Gene Name: Homo sapiens zinc finger CCHC-type containing 17 (ZCCHC17), transcript variant 3, mRNA.
• Database Link:
  NP_057589
  Entrez Gene 500555 RatEntrez Gene 619605 MouseEntrez Gene 51538 Human
  UniProt ID Q9NP64
• Synonyms: HSPC251; pNO40; PS1D