CCRK (CDK20) Mouse Monoclonal Antibody [Clone ID: OTI1B3]

CAT#: CF809548

Carrier-free (BSA/glycerol-free) CDK20 mouse monoclonal antibody,clone OTI1B3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK20 (RC214191, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK20 (1:2000). Positive lysates LY405956 (100ug) and LC405956 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1B3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human CDK20 (NP_848519) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.4 kDa
• Gene Name: Homo sapiens cyclin dependent kinase 20 (CDK20), transcript variant 1, mRNA.
• Database Link:
  NP_848519
  Entrez Gene 105278 MouseEntrez Gene 364666 RatEntrez Gene 23552 Human
  UniProt ID Q8IZL9
• Background: The protein encoded by this gene contains a kinase domain most closely related to the cyclin-dependent protein kinases. The encoded kinase may activate cyclin-dependent kinase 2 and is involved in cell growth. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Dec 2009]
• Synonyms: CCRK; CDCH; P42; PNQALRE

Reference Data

• Protein Families: Druggable Genome, Protein Kinase