ANP (NPPA) Mouse Monoclonal Antibody [Clone ID: OTI4H4]

CAT#: CF809559

Carrier-free (BSA/glycerol-free) NPPA mouse monoclonal antibody, clone OTI4H4

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPPA (RC203236, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPPA (1:2000). Positive lysates LY401862 (100ug) and LC401862 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from mouse heart tissue lysate by using anti-NPPA monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human adult heart tissue within the normal limits using anti-NPPA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809559) (1:500)

Specifications

Product Data

• Clone Name: OTI4H4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 26-153 of human NPPA(NP_006163) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13.6 kDa
• Gene Name: Homo sapiens natriuretic peptide A (NPPA), mRNA.
• Database Link:
  NP_006163
  Entrez Gene 4878 Human
  UniProt ID P01160
• Background: The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009]
• Synonyms: ANF; ANP; ATFB6; ATRST2; CDD; CDD-ANF; CDP; PND

Reference Data

• Protein Families: Secreted Protein, Transmembrane