LHX4 Mouse Monoclonal Antibody [Clone ID: OTI4E11]

CAT#: CF809561

Carrier-free (BSA/glycerol-free) LHX4 mouse monoclonal antibody,clone OTI4E11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LHX4 (RC204014, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LHX4 (1:2000). Positive lysates LY403244 (100ug) and LC403244 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from HepG2 by using anti-LHX4 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI4E11
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-260 of human LHX4 (NP_203129) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.9 kDa
• Gene Name: Homo sapiens LIM homeobox 4 (LHX4), mRNA.
• Database Link:
  NP_203129
  Entrez Gene 16872 MouseEntrez Gene 360858 RatEntrez Gene 89884 Human
  UniProt ID Q969G2
• Background: This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor involved in the control of differentiation and development of the pituitary gland. Mutations in this gene cause combined pituitary hormone deficiency 4. [provided by RefSeq, Dec 2010]
• Synonyms: CPHD4

Reference Data

• Protein Families: Druggable Genome, Transcription Factors